› 3l8u

X-ray diffraction
2Å resolution

Crystal structure of SMU.1707c, a putative rRNA methyltransferase from streptococcus mutans UA159

Released:
DOI: 10.2210/pdb3l8u/pdb
PDB entry 3l8u coloured by chain, front view.
PDB entry 3l8u coloured by chain, side view.
PDB entry 3l8u coloured by chain, top view.
Source organism: Streptococcus mutans UA159
Entry authors: Gao X-Z, Wang K-T, Su X-D

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative tRNA (cytidine(34)-2'-O)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 21.19 KDa
Source organism: Streptococcus mutans UA159
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DSR4 (Residues: 1-182; Coverage: 100%)
Gene name: SMU_1707c
Sequence domains: SpoU rRNA Methylase family
Structure domains: Alpha/beta knot

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P43
Unit cell:
a: 82.277Å b: 82.277Å c: 70.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.239
Expression system: Escherichia coli

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