› 3le0

X-ray diffraction
1.91Å resolution

Lectin Domain of Lectinolysin complexed with Glycerol

Released:
DOI: 10.2210/pdb3le0/pdb
PDB entry 3le0 coloured by chain, front view.
PDB entry 3le0 coloured by chain, side view.
PDB entry 3le0 coloured by chain, top view.
Source organism: Streptococcus mitis
Primary publication:
Structure of the lectin regulatory domain of the cholesterol-dependent cytolysin lectinolysin reveals the basis for its lewis antigen specificity.
Feil SC, Lawrence S, Mulhern TD, Holien JK, Hotze EM, Farrand S, Tweten RK, Parker MW
Structure 20 248-58 (2012)
PMID: 22325774

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol-activated cytolysin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.87 KDa
Source organism: Streptococcus mitis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2PHL4 (Residues: 38-190; Coverage: 24%)
Gene names: SMNM65_03180, samhpaf, sm-hpaf
Sequence domains: NedA-like, galactose-binding domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand NI 1 x NI
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P43212
Unit cell:
a: 67.189Å b: 67.189Å c: 98.322Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.206
Expression system: Escherichia coli

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Citations

11 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
10 more

5 mentions without citation

Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum.
Guyon et al. (2014)
4 more