› 3lfp

X-ray diffraction
2Å resolution

Crystal Structure of the Restriction-Modification Controller Protein C.Csp231I

Released:
DOI: 10.2210/pdb3lfp/pdb
PDB entry 3lfp coloured by chain, front view.
PDB entry 3lfp coloured by chain, side view.
PDB entry 3lfp coloured by chain, top view.
Source organism: Citrobacter sp. RFL231
Primary publication:
Structural analysis of a novel class of R-M controller proteins: C.Csp231I from Citrobacter sp. RFL231.
McGeehan JE, Streeter SD, Thresh SJ, Taylor JE, Shevtsov MB, Kneale GG
OpenAccess logo J Mol Biol 409 177-88 (2011)
PMID: 21440553

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.38 KDa
Source organism: Citrobacter sp. RFL231
Expression system: Escherichia coli
UniProt:
  • Canonical: Q32WH4 (Residues: 1-98; Coverage: 100%)
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: F4132
Unit cell:
a: 137.37Å b: 137.37Å c: 137.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.225
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Guide RNA Categorization Enables Target Site Choice in Tn7-CRISPR-Cas Transposons.
Petassi et al. (2020)
9 more

2 mentions without citation

Structural analysis of a novel class of R-M controller proteins: C.Csp231I from Citrobacter sp. RFL231.
McGeehan et al. (2011)
1 more