› 3lqr

X-ray diffraction
3.9Å resolution

Structure of CED-4:CED-3 complex

Released:
DOI: 10.2210/pdb3lqr/pdb
PDB entry 3lqr coloured by chain, front view.
PDB entry 3lqr coloured by chain, side view.
PDB entry 3lqr coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Crystal structure of the Caenorhabditis elegans apoptosome reveals an octameric assembly of CED-4.
Qi S, Pang Y, Hu Q, Liu Q, Li H, Zhou Y, He T, Liang Q, Liu Y, Yuan X, Luo G, Li H, Wang J, Yan N, Shi Y
Cell 141 446-57 (2010)
PMID: 20434985

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell death protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 62.95 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30429 (Residues: 1-571; Coverage: 96%)
  • Best match: P30429-2 (Residues: 1-549)
Gene names: C35D10.9, ced-4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: I422
Unit cell:
a: 181.33Å b: 181.33Å c: 202.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.255 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

Activation and regulation of the inflammasomes.
Latz et al. (2013)
31 more

3 mentions without citation

New insights into apoptosome structure and function.
Dorstyn et al. (2018)
2 more