› 3lu1

X-ray diffraction
2.5Å resolution

Crystal Structure Analysis of WbgU: a UDP-GalNAc 4-epimerase

Released:
DOI: 10.2210/pdb3lu1/pdb
PDB entry 3lu1 coloured by chain, front view.
PDB entry 3lu1 coloured by chain, side view.
PDB entry 3lu1 coloured by chain, top view.
Source organism: Plesiomonas shigelloides
Primary publication:
Altered architecture of substrate binding region defines the unique specificity of UDP-GalNAc 4-epimerases.
Bhatt VS, Guo CY, Guan W, Zhao G, Yi W, Liu ZJ, Wang PG
Protein Sci 20 856-66 (2011)
PMID: 21384454

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-galactosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 4-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 364 amino acids
Theoretical weight: 40.94 KDa
Source organism: Plesiomonas shigelloides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7BJX9 (Residues: 1-345; Coverage: 100%)
Gene name: wbgU
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
4 bound ligands:
Ligand UD2 4 x UD2
Ligand GLY 4 x GLY
Ligand SO4 3 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P32
Unit cell:
a: 78.13Å b: 78.13Å c: 231.924Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.204 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
6 other articles

1 mention without citation

Altered architecture of substrate binding region defines the unique specificity of UDP-GalNAc 4-epimerases.
Bhatt et al. (2011)