› 3lxi

X-ray diffraction
2.2Å resolution

Crystal Structure of Camphor-Bound CYP101D1

Released:
DOI: 10.2210/pdb3lxi/pdb
PDB entry 3lxi coloured by chain, front view.
PDB entry 3lxi coloured by chain, side view.
PDB entry 3lxi coloured by chain, top view.
Source organism: Novosphingobium aromaticivorans DSM 12444
Primary publication:
Molecular characterization of a class I P450 electron transfer system from Novosphingobium aromaticivorans DSM12444.
Yang W, Bell SG, Wang H, Zhou W, Hoskins N, Dale A, Bartlam M, Wong LL, Rao Z
J Biol Chem 285 27372-27384 (2010)
PMID: 20576606

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 421 amino acids
Theoretical weight: 47.34 KDa
Source organism: Novosphingobium aromaticivorans DSM 12444
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2GB12 (Residues: 1-421; Coverage: 100%)
Gene name: Saro_0514
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand CAM 2 x CAM
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6422
Unit cell:
a: 151.637Å b: 151.637Å c: 195.403Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.189 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cytochromes P450 as promising catalysts for biotechnological application: chances and limitations.
Bernhardt et al. (2014)
1 more

6 mentions without citation

Molecular characterization of a class I P450 electron transfer system from Novosphingobium aromaticivorans DSM12444.
Yang et al. (2010)
5 more