› 3m1y

X-ray diffraction
2.4Å resolution

Crystal Structure of a Phosphoserine phosphatase (SerB) from Helicobacter pylori

Released:
DOI: 10.2210/pdb3m1y/pdb
PDB entry 3m1y coloured by chain, front view.
PDB entry 3m1y coloured by chain, side view.
PDB entry 3m1y coloured by chain, top view.
Source organism: Helicobacter pylori
Entry authors: Syed Ibrahim B, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoserine phosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 217 amino acids
Theoretical weight: 24.57 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O25367 (Residues: 2-207; Coverage: 100%)
Gene name: HP_0652
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand CL 5 x CL
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 51.607Å b: 84.719Å c: 204.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.271
Expression system: Escherichia coli BL21(DE3)

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