› 3m20

X-ray diffraction
2.37Å resolution

Crystal structure of DmpI from Archaeoglobus fulgidus determined to 2.37 Angstroms resolution

Released:
DOI: 10.2210/pdb3m20/pdb
PDB entry 3m20 coloured by chain, front view.
PDB entry 3m20 coloured by chain, side view.
PDB entry 3m20 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Kinetic and structural characterization of DmpI from Helicobacter pylori and Archaeoglobus fulgidus, two 4-oxalocrotonate tautomerase family members.
Almrud JJ, Dasgupta R, Czerwinski RM, Kern AD, Hackert ML, Whitman CP
Bioorg Chem 38 252-9 (2010)
PMID: 20709352

Function and Biology Details

Reaction catalysed: 
Keto-oxaloacetate = enol-oxaloacetate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-oxalocrotonate tautomerase-like domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 62 amino acids
Theoretical weight: 6.83 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O29588 (Residues: 2-63; Coverage: 98%)
Gene name: AF_0669
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 49.07Å b: 49.07Å c: 118.747Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.296 0.296 0.316
Expression system: Escherichia coli

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Citations

1 review citation

Small Proteins in Archaea, a Mainly Unexplored World.
Weidenbach et al. (2022)
1 other article

2 mentions without citation

Kinetic and structural characterization of DmpI from Helicobacter pylori and Archaeoglobus fulgidus, two 4-oxalocrotonate tautomerase family members.
Almrud et al. (2010)
1 more