› 3m4c

X-ray diffraction
1.9Å resolution

A Zn-mediated tetrahedral protein lattice cage encapsulating a microperoxidase

Released:
DOI: 10.2210/pdb3m4c/pdb
PDB entry 3m4c coloured by chain, front view.
PDB entry 3m4c coloured by chain, side view.
PDB entry 3m4c coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of a microperoxidase inside a metal-directed protein cage.
Ni TW, Tezcan FA
Angew Chem Int Ed Engl 49 7014-8 (2010)
PMID: 20721993

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142155 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562
Heme-Peptide Fragment Chain: E
Molecule details ›
Chain: E
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand HEM 5 x HEM
Ligand ACT 4 x ACT
Ligand ZN 10 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: R32
Unit cell:
a: 126.892Å b: 126.892Å c: 167.944Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.187 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Interfacial metal coordination in engineered protein and peptide assemblies.
Sontz et al. (2014)
4 more

3 mentions without citation

Design and engineering of artificial oxygen-activating metalloenzymes.
Nastri et al. (2016)
2 more