› 3m8f

X-ray diffraction
2.8Å resolution

Protein structure of type III plasmid segregation TubR mutant

Released:
DOI: 10.2210/pdb3m8f/pdb
PDB entry 3m8f coloured by chain, front view.
PDB entry 3m8f coloured by chain, side view.
PDB entry 3m8f coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar israelensis
Primary publication:
Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition.
Ni L, Xu W, Kumaraswami M, Schumacher MA
Proc Natl Acad Sci U S A 107 11763-8 (2010)
PMID: 20534443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional repressor TubR Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 14.11 KDa
Source organism: Bacillus thuringiensis serovar israelensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KNP2 (Residues: 1-104; Coverage: 100%)
Gene names: ATN07_33545, pBt157, tubR
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 104.4Å b: 79.518Å c: 32.351Å
α: 90° β: 102.06° γ: 90°
R-values:
R R work R free
0.207 0.204 0.266
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The evolution of the cytoskeleton.
Wickstead et al. (2011)
9 more

1 mention without citation

Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition.
Ni et al. (2010)