› 3m9a

X-ray diffraction
2.5Å resolution

Protein structure of type III plasmid segregation TubR

Released:
DOI: 10.2210/pdb3m9a/pdb
PDB entry 3m9a coloured by chain, front view.
PDB entry 3m9a coloured by chain, side view.
PDB entry 3m9a coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar israelensis
Primary publication:
Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition.
Ni L, Xu W, Kumaraswami M, Schumacher MA
Proc Natl Acad Sci U S A 107 11763-8 (2010)
PMID: 20534443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional repressor TubR Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.01 KDa
Source organism: Bacillus thuringiensis serovar israelensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KNP2 (Residues: 1-104; Coverage: 100%)
Gene names: ATN07_33545, pBt157, tubR
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I222
Unit cell:
a: 32.494Å b: 69.546Å c: 114.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.219 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The evolution of the cytoskeleton.
Wickstead et al. (2011)
9 more

2 mentions without citation

Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition.
Ni et al. (2010)
1 more