› 3mej

X-ray diffraction
2.49Å resolution

Crystal structure of putative transcriptional regulator ywtF from Bacillus subtilis, Northeast Structural Genomics Consortium Target SR736

Released:
DOI: 10.2210/pdb3mej/pdb
PDB entry 3mej coloured by chain, front view.
PDB entry 3mej coloured by chain, side view.
PDB entry 3mej coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kuzin A, Su M, Seetharaman J, Mao M, Xiao R, Ciccosanti C, Lee D, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagT Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 32.34 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WY78 (Residues: 46-322; Coverage: 86%)
Gene names: BSU35840, tagT, ywtF
Sequence domains: LytR_cpsA_psr family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P41212
Unit cell:
a: 66.21Å b: 66.21Å c: 141.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.206 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links