› 3mkh

X-ray diffraction
2Å resolution

Podospora anserina Nitroalkane Oxidase

Released:
DOI: 10.2210/pdb3mkh/pdb
PDB entry 3mkh coloured by chain, front view.
PDB entry 3mkh coloured by chain, side view.
PDB entry 3mkh coloured by chain, top view.
Source organism: Podospora anserina
Primary publication:
Identification of a hypothetical protein from Podospora anserina as a nitroalkane oxidase.
Tormos JR, Taylor AB, Daubner SC, Hart PJ, Fitzpatrick PF
Biochemistry 49 5035-41 (2010)
PMID: 20481475

Function and Biology Details

Reaction catalysed: 
A nitroalkane + H(2)O + O(2) = an aldehyde or ketone + nitrite + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroalkane oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 438 amino acids
Theoretical weight: 46.47 KDa
Source organism: Podospora anserina
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2AM55 (Residues: 1-430; Coverage: 100%)
Gene names: PODANS_5_6340, Pa_5_6340
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
2 bound ligands:
Ligand MG 2 x MG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43
Unit cell:
a: 137.501Å b: 137.501Å c: 131.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Flavoprotein oxidases: classification and applications.
Dijkman et al. (2013)
2 more

2 mentions without citation

Identification of a hypothetical protein from Podospora anserina as a nitroalkane oxidase.
Tormos et al. (2010)
1 more