› 3mkv

X-ray diffraction
2.4Å resolution

Crystal structure of amidohydrolase eaj56179

Released:
DOI: 10.2210/pdb3mkv/pdb
PDB entry 3mkv coloured by chain, front view.
PDB entry 3mkv coloured by chain, side view.
PDB entry 3mkv coloured by chain, top view.
Source organism: unidentified
Primary publication:
Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily .
Xiang DF, Patskovsky Y, Xu C, Fedorov AA, Fedorov EV, Sisco AA, Sauder JM, Burley SK, Almo SC, Raushel FM
Biochemistry 49 6791-803 (2010)
PMID: 20604542

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 426 amino acids
Theoretical weight: 45.71 KDa
Source organism: unidentified
Expression system: Escherichia coli
UniProt:
  • Canonical: B2T4I1 (Residues: 1-414; Coverage: 100%)
Gene name: Bphyt_2089
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 16 x ZN
Ligand SO4 13 x SO4
Ligand GOL 2 x GOL
Ligand CO3 2 x CO3
1 modified residue:
Ligand KCX 8 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 92.022Å b: 198.599Å c: 104.294Å
α: 90° β: 107.76° γ: 90°
R-values:
R R work R free
0.173 0.171 0.229
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Structure-based function discovery of an enzyme for the hydrolysis of phosphorylated sugar lactones.
Xiang et al. (2012)
6 more

5 mentions without citation

Gulosibacter molinativorax ON4T molinate hydrolase, a novel cobalt-dependent amidohydrolase.
Duarte et al. (2011)
4 more