› 3mlg

X-ray diffraction
2.29Å resolution

2ouf-2x, a designed knotted protein

Released:
DOI: 10.2210/pdb3mlg/pdb
PDB entry 3mlg coloured by chain, front view.
PDB entry 3mlg coloured by chain, side view.
PDB entry 3mlg coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structure and folding of a designed knotted protein.
King NP, Jacobitz AW, Sawaya MR, Goldschmidt L, Yeates TO
Proc Natl Acad Sci U S A 107 20732-7 (2010)
PMID: 21068371

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-127704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF2018 family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.96 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O25025 (Residues: 1-92, 12-92; Coverage: 98%)
Gene name: HP_0242
Sequence domains: Domain of unknown function (DUF2018)
Structure domains: Knotted protein, ribbon-helix-helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 44.942Å b: 75.39Å c: 118.573Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.248 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Molecular Knots.
Fielden et al. (2017)
12 more

4 mentions without citation

Structural analysis of hypothetical proteins from Helicobacter pylori: an approach to estimate functions of unknown or hypothetical proteins.
Park et al. (2012)
3 more