› 3mln

X-ray diffraction
2.4Å resolution

DNA binding domain of Early B-cell Factor 1 (Ebf1) bound to DNA (crystal form II)

Released:
DOI: 10.2210/pdb3mln/pdb
PDB entry 3mln coloured by chain, front view.
PDB entry 3mln coloured by chain, side view.
PDB entry 3mln coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins.
Treiber N, Treiber T, Zocher G, Grosschedl R
Genes Dev 24 2270-5 (2010)
PMID: 20876732

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-116578 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transcription factor COE1 Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 224 amino acids
Theoretical weight: 25.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07802 (Residues: 24-241; Coverage: 37%)
Gene names: Coe1, Ebf, Ebf1
Sequence domains: Transcription factor COE1 DNA-binding domain
DNA (5'-D(*CP*TP*TP*TP*AP*TP*TP*CP*CP*CP*AP*TP*GP*GP*GP*AP*AP*TP*AP*AP*AP*G)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 71.2Å b: 100.7Å c: 72Å
α: 90° β: 101.2° γ: 90°
R-values:
R R work R free
0.173 0.171 0.219
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

The regulatory network of B-cell differentiation: a focused view of early B-cell factor 1 function.
Boller et al. (2014)
2 more

2 mentions without citation

Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins.
Treiber et al. (2010)
1 more