› 3mua

X-ray diffraction
1.5Å resolution

Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus.

Released:
DOI: 10.2210/pdb3mua/pdb
PDB entry 3mua coloured by chain, front view.
PDB entry 3mua coloured by chain, side view.
PDB entry 3mua coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Solomon V, Zolotnitsky G, Alhadeff R, Shoham Y, Shoham G

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 38.7 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09LY9 (Residues: 1-331; Coverage: 100%)
Gene name: xynA2
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
4 bound ligands:
Ligand XYP 1 x XYP
Ligand NA 2 x NA
Ligand ACT 2 x ACT
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2
Unit cell:
a: 169.228Å b: 80.771Å c: 79.056Å
α: 90° β: 91.73° γ: 90°
R-values:
R R work R free
0.192 0.192 0.202
Expression system: Escherichia coli

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