› 3n0i

X-ray diffraction
1.65Å resolution

Crystal Structure of Ad37 fiber knob in complex with GD1a oligosaccharide

Released:
DOI: 10.2210/pdb3n0i/pdb
PDB entry 3n0i coloured by chain, front view.
PDB entry 3n0i coloured by chain, side view.
PDB entry 3n0i coloured by chain, top view.
Source organism: Human adenovirus D37
Primary publication:
The GD1a glycan is a cellular receptor for adenoviruses causing epidemic keratoconjunctivitis.
Nilsson EC, Storm RJ, Bauer J, Johansson SM, Lookene A, Ångström J, Hedenström M, Eriksson TL, Frängsmyr L, Rinaldi S, Willison HJ, Pedrosa Domellöf F, Stehle T, Arnberg N
Nat Med 17 105-9 (2011)
PMID: 21151139

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Adenoviral fibre protein knob domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 194 amino acids
Theoretical weight: 21.72 KDa
Source organism: Human adenovirus D37
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q64823 (Residues: 177-365; Coverage: 52%)
Gene name: L5
Sequence domains: Adenoviral fibre protein (knob domain)
Structure domains: Adenovirus pIV-related, attachment domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, NGA, SIA
Carbohydrate polymer
1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 60.99Å b: 69.77Å c: 74.9Å
α: 90° β: 94.92° γ: 90°
R-values:
R R work R free
0.16 0.16 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

54 review citations

Adenovirus infections in immunocompetent and immunocompromised patients.
Lion T. (2014)
53 more

5 mentions without citation

The sweet spot: defining virus-sialic acid interactions.
Stencel-Baerenwald et al. (2014)
4 more