› 3nbm

X-ray diffraction
1.3Å resolution

The lactose-specific IIB component domain structure of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) from Streptococcus pneumoniae.

Released:
DOI: 10.2210/pdb3nbm/pdb
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PDB entry 3nbm coloured by chain, front view.
PDB entry 3nbm coloured by chain, side view.
PDB entry 3nbm coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry authors: Cuff ME, Chhor G, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system lactose-specific EIICB component Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 11.97 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UNS1 (Residues: 453-557; Coverage: 19%)
Gene names: SP_0478, lacE-1
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P43212
Unit cell:
a: 41.372Å b: 41.372Å c: 98.527Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.167
Expression system: Escherichia coli

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