› 3ned

X-ray diffraction
0.95Å resolution

mRouge

Released:
DOI: 10.2210/pdb3ned/pdb
Model geometry
Fit model/data
PDB entry 3ned coloured by chain, front view.
PDB entry 3ned coloured by chain, side view.
PDB entry 3ned coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Generation of longer emission wavelength red fluorescent proteins using computationally designed libraries.
Chica RA, Moore MM, Allen BD, Mayo SL
Proc Natl Acad Sci U S A 107 20257-62 (2010)
PMID: 21059931

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.75 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1MPT3 (Residues: 2-236; Coverage: 100%)
Gene names: PAmCherry, PAmCherry1
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand ACT 1 x ACT
3 modified residues:
Ligand CH6 1 x CH6
Ligand EYG 1 x EYG
Ligand NRQ 1 x NRQ

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 48.807Å b: 42.76Å c: 61.203Å
α: 90° β: 112.35° γ: 90°
R-values:
R R work R free
0.135 0.133 0.157
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
8 more

4 mentions without citation

Streamlining the interface between electronics and neural systems for bidirectional electrochemical communication.
Cho et al. (2023)
3 more