› 3nes

X-ray diffraction
1.75Å resolution

V30M mutant human transthyretin (TTR) complexed with GC-1 (V30M:GC-1)

Released:
DOI: 10.2210/pdb3nes/pdb
PDB entry 3nes coloured by chain, front view.
PDB entry 3nes coloured by chain, side view.
PDB entry 3nes coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The binding of synthetic triiodo l-thyronine analogs to human transthyretin: molecular basis of cooperative and non-cooperative ligand recognition.
Trivella DB, Sairre MI, Foguel D, Lima LM, Polikarpov I
J Struct Biol 173 323-32 (2011)
PMID: 20937391

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transthyretin Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 12.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02766 (Residues: 30-145; Coverage: 91%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

2 bound ligands:
Ligand B72 2 x B72
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21212
Unit cell:
a: 43.731Å b: 85.451Å c: 64.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

The flavonoid luteolin, but not luteolin-7-O-glucoside, prevents a transthyretin mediated toxic response.
Iakovleva et al. (2015)
3 more

1 mention without citation

Chemical Genetic Analysis and Functional Characterization of Staphylococcal Wall Teichoic Acid 2-Epimerases Reveals Unconventional Antibiotic Drug Targets.
Mann et al. (2016)