› 3nfz

X-ray diffraction
2.15Å resolution

Crystal structure of murine aminoacylase 3 in complex with N-acetyl-L-tyrosine

Released:
DOI: 10.2210/pdb3nfz/pdb
PDB entry 3nfz coloured by chain, front view.
PDB entry 3nfz coloured by chain, side view.
PDB entry 3nfz coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structures of aminoacylase 3 in complex with acetylated substrates.
Hsieh JM, Tsirulnikov K, Sawaya MR, Magilnick N, Abuladze N, Kurtz I, Abramson J, Pushkin A
Proc Natl Acad Sci U S A 107 17962-7 (2010)
PMID: 20921362

Function and Biology Details

Reaction catalysed: 
An N-acetyl-L-cysteine-S-conjugate + H(2)O = an L-cysteine-S-conjugate + acetate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming) Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q91XE4 (Residues: 1-318; Coverage: 100%)
Gene names: Acy3, Aspa2
Sequence domains: Succinylglutamate desuccinylase / Aspartoacylase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 2 x CL
Ligand 3NF 1 x 3NF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P62
Unit cell:
a: 93.498Å b: 93.498Å c: 98.037Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.197
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Carboxypeptidases in disease: insights from peptidomic studies.
Sapio et al. (2014)
6 other articles

1 mention without citation

Structures of aminoacylase 3 in complex with acetylated substrates.
Hsieh et al. (2010)