› 3ngy

X-ray diffraction
2.2Å resolution

Crystal structure of RNase T (E92G mutant)

Released:
DOI: 10.2210/pdb3ngy/pdb
PDB entry 3ngy coloured by chain, front view.
PDB entry 3ngy coloured by chain, side view.
PDB entry 3ngy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation.
Hsiao YY, Yang CC, Lin CL, Lin JL, Duh Y, Yuan HS
Nat Chem Biol 7 236-43 (2011)
PMID: 21317904

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-151607 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease T Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 25.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P30014 (Residues: 1-215; Coverage: 100%)
Gene names: JW1644, b1652, rnt
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H
his tag sequence Chain: E
Molecule details ›
Chain: E
Length: 6 amino acids
Theoretical weight: 796 Da
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CO 3 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 58.763Å b: 107.718Å c: 121.745Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.175 0.224
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial ribonucleases and their roles in RNA metabolism.
Bechhofer et al. (2019)
3 more

2 mentions without citation

How an exonuclease decides where to stop in trimming of nucleic acids: crystal structures of RNase T-product complexes.
Hsiao et al. (2012)
1 more