› 3nib

X-ray diffraction
2.7Å resolution

Teg14 Apo

Released:
DOI: 10.2210/pdb3nib/pdb
PDB entry 3nib coloured by chain, front view.
PDB entry 3nib coloured by chain, side view.
PDB entry 3nib coloured by chain, top view.
Source organism: uncultured soil bacterium
Primary publication:
The 2.7 Å resolution structure of the glycopeptide sulfotransferase Teg14.
Bick MJ, Banik JJ, Darst SA, Brady SF
Acta Crystallogr D Biol Crystallogr 66 1278-86 (2010)
PMID: 21123867

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 34.48 KDa
Source organism: uncultured soil bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7T1D9 (Residues: 1-275; Coverage: 100%)
Gene name: teg14
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand NHE 1 x NHE
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3121
Unit cell:
a: 112.194Å b: 112.194Å c: 74.807Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glycopeptide antibiotic biosynthesis.
Yim et al. (2014)
1 more

2 mentions without citation

Crystal structure of the catalytic domain of Clostridium perfringens neuraminidase in complex with a non-carbohydrate-based inhibitor, 2-(cyclohexylamino)ethanesulfonic acid.
Lee et al. (2017)
1 more