› 3nmj

X-ray diffraction
3.1Å resolution

Crystal structure of a nickel mediated dimer for the phenanthroline-modified cytochrome cb562 variant, MBP-Phen2

Released:
DOI: 10.2210/pdb3nmj/pdb
PDB entry 3nmj coloured by chain, front view.
PDB entry 3nmj coloured by chain, side view.
PDB entry 3nmj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Porous protein frameworks with unsaturated metal centers in sterically encumbered coordination sites.
Radford RJ, Lawrenz M, Nguyen PC, McCammon JA, Tezcan FA
Chem Commun (Camb) 47 313-5 (2011)
PMID: 20740227

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

3 bound ligands:
Ligand HEM 4 x HEM
Ligand PXX 4 x PXX
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P65
Unit cell:
a: 99.291Å b: 99.291Å c: 109.317Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.238
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metal-biomolecule frameworks (MBioFs).
Imaz et al. (2011)
1 more