› 3nok

X-ray diffraction
1.65Å resolution

Crystal structure of Myxococcus xanthus Glutaminyl Cyclase

Released:
DOI: 10.2210/pdb3nok/pdb
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Source organism: Myxococcus xanthus
Primary publication:
Kinetic and structural characterization of bacterial glutaminyl cyclases from Zymomonas mobilis and Myxococcus xanthus.
Carrillo DR, Parthier C, Jänckel N, Grandke J, Stelter M, Schilling S, Boehme M, Neumann P, Wolf R, Demuth HU, Stubbs MT, Rahfeld JU
Biol Chem 391 1419-28 (2010)
PMID: 20868223

Function and Biology Details

Reaction catalysed: 
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminyl Cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 29.41 KDa
Source organism: Myxococcus xanthus
Expression system: Escherichia coli
UniProt:
  • Canonical: E7FH78 (Residues: 1-248; Coverage: 100%)
Sequence domains: Glutamine cyclotransferase

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand MES 2 x MES
Ligand DDQ 2 x DDQ
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 65.79Å b: 46.06Å c: 81Å
α: 90° β: 112.97° γ: 90°
R-values:
R R work R free
0.197 0.195 0.236
Expression system: Escherichia coli

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Citations

2 review citations

Pyroglutamate Aβ cascade as drug target in Alzheimer's disease.
Bayer TA. (2022)
1 more

2 mentions without citation

Significant reduction in errors associated with nonbonded contacts in protein crystal structures: automated all-atom refinement with PrimeX.
Bell et al. (2012)
1 more

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