› 3nvs

X-ray diffraction
1.02Å resolution

1.02 Angstrom resolution crystal structure of 3-phosphoshikimate 1-carboxyvinyltransferase from Vibrio cholerae in complex with shikimate-3-phosphate (partially photolyzed) and glyphosate

Released:
DOI: 10.2210/pdb3nvs/pdb
PDB entry 3nvs coloured by chain, front view.
PDB entry 3nvs coloured by chain, side view.
PDB entry 3nvs coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Minasov G, Light SH, Halavaty A, Shuvalova G, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoshikimate 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 48.89 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KRB0 (Residues: 1-426; Coverage: 100%)
Gene names: VC_1732, aroA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

7 bound ligands:
Ligand S3P 1 x S3P
Ligand SKM 1 x SKM
Ligand GPJ 1 x GPJ
Ligand MG 3 x MG
Ligand CL 4 x CL
Ligand EPE 1 x EPE
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 43.866Å b: 87.484Å c: 49.994Å
α: 90° β: 105.26° γ: 90°
R-values:
R R work R free
0.115 0.114 0.138
Expression system: Escherichia coli BL21(DE3)

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