3o04

X-ray diffraction
1.85Å resolution

Crystal structure of the beta-keto-acyl carrier protein synthase II (lmo2201) from Listeria monocytogenes

Released:
Source organism: Listeria monocytogenes EGD-e
Entry authors: Anderson SM, Wawrzak Z, Onopriyenko O, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-186952 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 44.96 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Y574 (Residues: 1-413; Coverage: 100%)
Gene name: lmo2201
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P43212
Unit cell:
a: 74.742Å b: 74.742Å c: 170.694Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.175
Expression system: Escherichia coli BL21(DE3)