› 3o26

X-ray diffraction
1.91Å resolution

The structure of salutaridine reductase from Papaver somniferum.

Released:
DOI: 10.2210/pdb3o26/pdb
PDB entry 3o26 coloured by chain, front view.
PDB entry 3o26 coloured by chain, side view.
PDB entry 3o26 coloured by chain, top view.
Source organism: Papaver somniferum
Primary publication:
Atomic structure of salutaridine reductase from the opium poppy (Papaver somniferum).
Higashi Y, Kutchan TM, Smith TJ
J Biol Chem 286 6532-41 (2011)
PMID: 21169353

Function and Biology Details

Reaction catalysed: 
Salutaridinol + NADP(+) = salutaridine + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Salutaridine reductase Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 34.08 KDa
Source organism: Papaver somniferum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q071N0 (Residues: 1-311; Coverage: 100%)
Gene name: SALR
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6422
Unit cell:
a: 139.906Å b: 139.906Å c: 100.174Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.193 0.208
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Benzylisoquinoline alkaloid metabolism: a century of discovery and a brave new world.
Hagel et al. (2013)
1 more

6 mentions without citation

Atomic structure of salutaridine reductase from the opium poppy (Papaver somniferum).
Higashi et al. (2011)
5 more