› 3o2a

X-ray diffraction
1.9Å resolution

Ligand-binding domain of GluA2 (flip) ionotropic glutamate receptor in complex with an allosteric modulator

Released:
DOI: 10.2210/pdb3o2a/pdb
PDB entry 3o2a coloured by chain, front view.
PDB entry 3o2a coloured by chain, side view.
PDB entry 3o2a coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A novel series of positive modulators of the AMPA receptor: discovery and structure based hit-to-lead studies.
Jamieson C, Basten S, Campbell RA, Cumming IA, Gillen KJ, Gillespie J, Kazemier B, Kiczun M, Lamont Y, Lyons AJ, Maclean JK, Moir EM, Morrow JA, Papakosta M, Rankovic Z, Smith L
Bioorg Med Chem Lett 20 5753-6 (2010)
PMID: 20805031

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.18 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
  • Best match: P19491-2 (Residues: 413-527, 653-796)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

4 bound ligands:
Ligand O30 1 x O30
Ligand GLU 1 x GLU
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 64.338Å b: 90.398Å c: 47.435Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Structure and mechanism of glutamate receptor ion channel assembly, activation and modulation.
Mayer ML. (2011)
6 more