› 3ocl

X-ray diffraction
2.3Å resolution

Crystal structure of penicillin-binding protein 3 from Pseudomonas aeruginosa in complex with carbenicillin

Released:
DOI: 10.2210/pdb3ocl/pdb
PDB entry 3ocl coloured by chain, front view.
PDB entry 3ocl coloured by chain, side view.
PDB entry 3ocl coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structures of penicillin-binding protein 3 from Pseudomonas aeruginosa: comparison of native and antibiotic-bound forms.
Sainsbury S, Bird L, Rao V, Shepherd SM, Stuart DI, Hunter WN, Owens RJ, Ren J
OpenAccess logo J Mol Biol 405 173-84 (2011)
PMID: 20974151

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan D,D-transpeptidase FtsI Chain: A
Molecule details ›
Chain: A
Length: 564 amino acids
Theoretical weight: 61.15 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51504 (Residues: 35-579; Coverage: 94%)
Gene names: ALP65_00912, CAZ10_21230, GNQ48_11285, GUL26_13375, IPC1295_15535, IPC737_20650, L4V69_30060, PAERUG_P19_London_7_VIM_2_05_10_00246, ftsI, ftsI_1, pbpB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand CB9 1 x CB9
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 69.475Å b: 83.435Å c: 89.845Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.248
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Glycosyltransferases and Transpeptidases/Penicillin-Binding Proteins: Valuable Targets for New Antibacterials.
Sauvage et al. (2016)
7 more

7 mentions without citation

Crystal structures of penicillin-binding protein 3 from Pseudomonas aeruginosa: comparison of native and antibiotic-bound forms.
Sainsbury et al. (2011)
6 more