3oeb

X-ray diffraction
1.55Å resolution

Crystal structure of the Q121E mutant of C.polysaccharolyticus CBM16-1 bound to mannopentaose

Released:

Function and Biology Details

Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-195599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
SLH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 15.83 KDa
Source organism: Caldanaerobius polysaccharolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZA17 (Residues: 614-756; Coverage: 13%)
Gene name: celA
Sequence domains: Carbohydrate binding domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, BMA
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 56.44Å b: 96.516Å c: 72.935Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.218
Expression system: Escherichia coli BL21(DE3)