› 3og8

X-ray diffraction
2.4Å resolution

Crystal structure of human RIG-I CTD bound to a 14-bp blunt-ended dsRNA

Released:
DOI: 10.2210/pdb3og8/pdb
PDB entry 3og8 coloured by chain, front view.
PDB entry 3og8 coloured by chain, side view.
PDB entry 3og8 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of RIG-I C-terminal domain bound to blunt-ended double-strand RNA without 5' triphosphate.
Lu C, Ranjith-Kumar CT, Hao L, Kao CC, Li P
OpenAccess logo Nucleic Acids Res 39 1565-75 (2011)
PMID: 20961956

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523093 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Antiviral innate immune response receptor RIG-I Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 15.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95786 (Residues: 802-925; Coverage: 13%)
Gene names: DDX58, RIGI
Sequence domains: C-terminal domain of RIG-I
Structure domains: RIG-I-like receptor, C-terminal regulatory domain
RNA (5'-R(*GP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 nucleotides
Theoretical weight: 4.51 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 36.99Å b: 70.3Å c: 125.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Immune signaling by RIG-I-like receptors.
Loo et al. (2011)
11 more

14 mentions without citation

Structural insights into RNA recognition and activation of RIG-I-like receptors.
Leung et al. (2012)
13 more