› 3ogj

X-ray diffraction
2.75Å resolution

Crystal structure of partial apo (92-227) of cGMP-dependent protein kinase

Released:
DOI: 10.2210/pdb3ogj/pdb
PDB entry 3ogj coloured by chain, front view.
PDB entry 3ogj coloured by chain, side view.
PDB entry 3ogj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Co-crystal structures of PKG Iβ (92-227) with cGMP and cAMP reveal the molecular details of cyclic-nucleotide binding.
Kim JJ, Casteel DE, Huang G, Kwon TH, Ren RK, Zwart P, Headd JJ, Brown NG, Chow DC, Palzkill T, Kim C
OpenAccess logo PLoS One 6 e18413 (2011)
PMID: 21526164

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-171845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 15.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13976 (Residues: 84-212; Coverage: 19%)
  • Best match: Q13976-2 (Residues: 92-227)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand CMP 2 x CMP
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43
Unit cell:
a: 62.588Å b: 62.588Å c: 199.522Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

IRAG and novel PKG targeting in the cardiovascular system.
Schlossmann et al. (2011)
3 more

5 mentions without citation

Advances, interactions, and future developments in the CNS, Phenix, and Rosetta structural biology software systems.
Adams et al. (2013)
4 more