› 3ohx

X-ray diffraction
3.5Å resolution

Molecular Basis for Complement Recognition and Inhibition Determined by Crystallographic Studies of the Staphylococcal Complement Inhibitor (SCIN) Bound to C3c and C3b

Released:
DOI: 10.2210/pdb3ohx/pdb
PDB entry 3ohx coloured by chain, front view.
PDB entry 3ohx coloured by chain, side view.
PDB entry 3ohx coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for complement recognition and inhibition determined by crystallographic studies of the staphylococcal complement inhibitor (SCIN) bound to C3c and C3b.
Garcia BL, Ramyar KX, Tzekou A, Ricklin D, McWhorter WJ, Lambris JD, Geisbrecht BV
J Mol Biol 402 17-29 (2010)
PMID: 20654625

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133825 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement C3 beta chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 23-667; Coverage: 39%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Complement C3c alpha' chain fragment 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 206 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 749-954; Coverage: 13%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Complement C3c alpha' chain fragment 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 343 amino acids
Theoretical weight: 39.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 1321-1663; Coverage: 21%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Staphylococcal complement inhibitor Chains: M, P
Molecule details ›
Chains: M, P
Length: 88 amino acids
Theoretical weight: 10.06 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: Q931M7 (Residues: 32-116; Coverage: 100%)
Gene names: SAV1942, scn
Sequence domains: Staphylococcal complement inhibitor SCIN
Structure domains: Substrate Binding Domain Of Dnak; Chain:A; Domain 2

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 231.159Å b: 231.497Å c: 68.885Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.274 0.294
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Complement System Part II: Role in Immunity.
Merle et al. (2015)
4 more

4 mentions without citation

Complement component C3: A structural perspective and potential therapeutic implications.
Geisbrecht et al. (2022)
3 more