› 3oiu

X-ray diffraction
1.32Å resolution

H-RasQ61L with allosteric switch in the "on" state

Released:
DOI: 10.2210/pdb3oiu/pdb
PDB entry 3oiu coloured by chain, front view.
PDB entry 3oiu coloured by chain, side view.
PDB entry 3oiu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric modulation of Ras-GTP is linked to signal transduction through RAF kinase.
Buhrman G, Kumar VS, Cirit M, Haugh JM, Mattos C
J Biol Chem 286 3323-31 (2011)
PMID: 21098031

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GNP 1 x GNP
Ligand CA 3 x CA
Ligand MG 2 x MG
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R32
Unit cell:
a: 87.985Å b: 87.985Å c: 135.146Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.192
Expression system: Escherichia coli

Quick links

Citations

12 review citations

'Pathway drug cocktail': targeting Ras signaling based on structural pathways.
Nussinov et al. (2013)
11 more

7 mentions without citation

A comprehensive survey of Ras mutations in cancer.
Prior et al. (2012)
6 more