› 3oj6

X-ray diffraction
1.7Å resolution

Crystal structure of Blasticidin S Deaminase from Coccidioides Immitis

Released:
DOI: 10.2210/pdb3oj6/pdb
PDB entry 3oj6 coloured by chain, front view.
PDB entry 3oj6 coloured by chain, side view.
PDB entry 3oj6 coloured by chain, top view.
Source organism: Coccidioides immitis
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Blasticidin S + H(2)O = deaminohydroxyblasticidin S + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Blasticidin-S deaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 158 amino acids
Theoretical weight: 16.52 KDa
Source organism: Coccidioides immitis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CI02 (Residues: 1-137; Coverage: 100%)
Gene name: CIMG_08654
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand EDO 5 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 46.48Å b: 56.28Å c: 90.29Å
α: 90° β: 95.09° γ: 90°
R-values:
R R work R free
0.153 0.151 0.189
Expression system: Escherichia coli BL21(DE3)

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