› 3om2

X-ray diffraction
1.9Å resolution

Crystal structure of B. megaterium levansucrase mutant D257A

Released:
DOI: 10.2210/pdb3om2/pdb
PDB entry 3om2 coloured by chain, front view.
PDB entry 3om2 coloured by chain, side view.
PDB entry 3om2 coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Polysaccharide synthesis of the levansucrase SacB from Bacillus megaterium is controlled by distinct surface motifs.
Strube CP, Homann A, Gamer M, Jahn D, Seibel J, Heinz DW
J Biol Chem 286 17593-600 (2011)
PMID: 21454585

Function and Biology Details

Reaction catalysed: 
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Levansucrase Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 51.03 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: D5DC07 (Residues: 29-484; Coverage: 100%)
Gene names: BMD_1536, sacB
Sequence domains: Levansucrase/Invertase
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand CIT 2 x CIT
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 49.003Å b: 55.289Å c: 163.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.218
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Review of Levan polysaccharide: From a century of past experiences to future prospects.
Öner et al. (2016)
2 more

5 mentions without citation

Polysaccharide synthesis of the levansucrase SacB from Bacillus megaterium is controlled by distinct surface motifs.
Strube et al. (2011)
4 more