› 3om4

X-ray diffraction
1.75Å resolution

Crystal structure of B. megaterium levansucrase mutant K373A

Released:
DOI: 10.2210/pdb3om4/pdb
PDB entry 3om4 coloured by chain, front view.
PDB entry 3om4 coloured by chain, side view.
PDB entry 3om4 coloured by chain, top view.
Source organism: Priestia megaterium
Entry authors: Strube CP, Homann A, Gamer M, Jahn D, Seibel J, Heinz DW

Function and Biology Details

Reaction catalysed: 
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Levansucrase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 51.01 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: D5DC07 (Residues: 29-484; Coverage: 100%)
Gene names: BMD_1536, sacB
Sequence domains: Levansucrase/Invertase
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

5 bound ligands:
Ligand CA 4 x CA
Ligand PEG 1 x PEG
Ligand PGE 5 x PGE
Ligand SO4 6 x SO4
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 93.585Å b: 100.075Å c: 95.544Å
α: 90° β: 90.67° γ: 90°
R-values:
R R work R free
0.202 0.202 0.213
Expression system: Escherichia coli

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