3om5

X-ray diffraction
1.95Å resolution

Crystal structure of B. megaterium levansucrase mutant N252A

Released:

Function and Biology Details

Reaction catalysed:
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Levansucrase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 51.03 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: D5DC07 (Residues: 29-484; Coverage: 100%)
Gene names: BMD_1536, sacB
Sequence domains: Levansucrase/Invertase
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 93.368Å b: 100.186Å c: 95.195Å
α: 90° β: 90.68° γ: 90°
R-values:
R R work R free
0.182 0.181 0.208
Expression system: Escherichia coli