› 3omy

X-ray diffraction
1.3Å resolution

Crystal structure of the pED208 TraM N-terminal domain

Released:
DOI: 10.2210/pdb3omy/pdb
PDB entry 3omy coloured by chain, front view.
PDB entry 3omy coloured by chain, side view.
PDB entry 3omy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.
Wong JJ, Lu J, Edwards RA, Frost LS, Glover JN
OpenAccess logo Nucleic Acids Res 39 6775-88 (2011)
PMID: 21565799

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Relaxosome protein TraM Chains: A, B
Molecule details ›
Chains: A, B
Length: 52 amino acids
Theoretical weight: 6.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33788 (Residues: 1-52; Coverage: 41%)
Gene name: traM
Sequence domains: TraM protein, DNA-binding
Structure domains: TraM protein, DNA-binding

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P41212
Unit cell:
a: 54.069Å b: 54.069Å c: 67.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Assembly and mechanisms of bacterial type IV secretion machines.
Zechner et al. (2012)
8 more

1 mention without citation

Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.
Wong et al. (2011)