3onl

X-ray diffraction
2.2Å resolution

yeast Ent3_ENTH-Vti1p_Habc complex structure

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Epsin N-terminal homology domains bind on opposite sides of two SNAREs.
Proc Natl Acad Sci U S A 108 12277-82 (2011)
PMID: 21746902

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-155607 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epsin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 17.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47160 (Residues: 28-170; Coverage: 35%)
Gene names: ENT3, J2048, YJR125C
Sequence domains: ENTH domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
t-SNARE VTI1 Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04338 (Residues: 3-99; Coverage: 45%)
Gene names: VTI1, YM9646.10C, YMR197C
Sequence domains: Vesicle transport v-SNARE protein N-terminus
Structure domains: t-snare proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 58.683Å b: 82.351Å c: 95.953Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.269
Expression system: Escherichia coli BL21(DE3)