› 3opz

X-ray diffraction
3.4Å resolution

Crystal structure of trans-sialidase in complex with the Fab fragment of a neutralizing monoclonal IgG antibody

Released:
DOI: 10.2210/pdb3opz/pdb
PDB entry 3opz coloured by chain, front view.
PDB entry 3opz coloured by chain, side view.
PDB entry 3opz coloured by chain, top view.
Source organisms:
Primary publication:
Trypanosoma cruzi trans-sialidase in complex with a neutralizing antibody: structure/function studies towards the rational design of inhibitors.
Buschiazzo A, Muiá R, Larrieux N, Pitcovsky T, Mucci J, Campetella O
OpenAccess logo PLoS Pathog 8 e1002474 (2012)
PMID: 22241998

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212090 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sialidase domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 648 amino acids
Theoretical weight: 71.39 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q26966 (Residues: 2-635; Coverage: 99%)
Sequence domains:
Structure domains:
heavy chain of the Fab fragment of immunoglobulin G Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 222 amino acids
Theoretical weight: 23.87 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
light chain of the Fab fragment of immunoglobulin G Chains: L, M, N
Molecule details ›
Chains: L, M, N
Length: 213 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand DIO 2 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P31
Unit cell:
a: 178.14Å b: 178.14Å c: 140.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.164 0.205
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Sialic acid metabolism and sialyltransferases: natural functions and applications.
Li et al. (2012)
8 more

3 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
2 more