› 3ozf

X-ray diffraction
1.94Å resolution

Crystal Structure of Plasmodium falciparum Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase in complex with hypoxanthine

Released:
DOI: 10.2210/pdb3ozf/pdb
PDB entry 3ozf coloured by chain, front view.
PDB entry 3ozf coloured by chain, side view.
PDB entry 3ozf coloured by chain, top view.
Source organism: Plasmodium falciparum FCR-3/Gambia
Primary publication:
Acyclic immucillin phosphonates: second-generation inhibitors of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase.
Hazleton KZ, Ho MC, Cassera MB, Clinch K, Crump DR, Rosario I, Merino EF, Almo SC, Tyler PC, Schramm VL
Chem Biol 19 721-30 (2012)
PMID: 22726686

Function and Biology Details

Reactions catalysed: 
XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148823 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine-xanthine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 250 amino acids
Theoretical weight: 28.41 KDa
Source organism: Plasmodium falciparum FCR-3/Gambia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20035 (Residues: 1-231; Coverage: 100%)
Gene name: LACZ
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand HPA 4 x HPA
Ligand POP 4 x POP
Ligand PO4 4 x PO4
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 75.035Å b: 88.907Å c: 80.371Å
α: 90° β: 117.06° γ: 90°
R-values:
R R work R free
0.192 0.189 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Prodrugs of phosphonates and phosphates: crossing the membrane barrier.
Wiemer et al. (2015)
5 more

1 mention without citation

Acyclic immucillin phosphonates: second-generation inhibitors of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase.
Hazleton et al. (2012)