› 3p22

X-ray diffraction
2.5Å resolution

Crystal structure of the ENE, a viral RNA stability element, in complex with A9 RNA

Released:
DOI: 10.2210/pdb3p22/pdb
PDB entry 3p22 coloured by chain, front view.
PDB entry 3p22 coloured by chain, side view.
PDB entry 3p22 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Poly(A) tail recognition by a viral RNA element through assembly of a triple helix.
Mitton-Fry RM, DeGregorio SJ, Wang J, Steitz TA, Steitz JA
Science 330 1244-7 (2010)
PMID: 21109672

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196713 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
Core ENE hairpin from KSHV PAN RNA Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 40 nucleotides
Theoretical weight: 12.91 KDa
Source organism: Synthetic construct
Expression system: Not provided
oligo(A)9 RNA Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 9 nucleotides
Theoretical weight: 2.92 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GTP 4 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 144.746Å b: 50.869Å c: 91.38Å
α: 90° β: 125.06° γ: 90°
R-values:
R R work R free
0.219 0.218 0.233
Expression system: Not provided

Quick links

Citations

32 review citations

lincRNAs: genomics, evolution, and mechanisms.
Ulitsky et al. (2013)
31 more

147 mentions without citation

Osteogenesis imperfecta.
Forlino et al. (2016)
146 more