› 3p9c

X-ray diffraction
1.8Å resolution

Crystal structure of perennial ryegrass LpOMT1 bound to SAH

Released:
DOI: 10.2210/pdb3p9c/pdb
PDB entry 3p9c coloured by chain, front view.
PDB entry 3p9c coloured by chain, side view.
PDB entry 3p9c coloured by chain, top view.
Source organism: Lolium perenne
Primary publication:
Structure-function analyses of a caffeic acid O-methyltransferase from perennial ryegrass reveal the molecular basis for substrate preference.
Louie GV, Bowman ME, Tu Y, Mouradov A, Spangenberg G, Noel JP
Plant Cell 22 4114-27 (2010)
PMID: 21177481

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195710 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caffeic acid O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 39.15 KDa
Source organism: Lolium perenne
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZTU2 (Residues: 1-360; Coverage: 100%)
Gene name: OMT1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
3 bound ligands:
Ligand DTV 1 x DTV
Ligand ACT 1 x ACT
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 67.699Å b: 67.699Å c: 249.794Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
4 more

3 mentions without citation

Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.
Lashley et al. (2022)
2 more