› 3p9x

X-ray diffraction
1.9Å resolution

Crystal structure of phosphoribosylglycinamide formyltransferase from Bacillus Halodurans

Released:
DOI: 10.2210/pdb3p9x/pdb
PDB entry 3p9x coloured by chain, front view.
PDB entry 3p9x coloured by chain, side view.
PDB entry 3p9x coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry authors: Patskovsky Y, Toro R, Foti R, Seidel RD, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191855 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.45 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KF54 (Residues: 1-188; Coverage: 100%)
Gene name: purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 68.795Å b: 68.662Å c: 128.938Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.226
Expression system: Escherichia coli

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