3pge

X-ray diffraction
2.8Å resolution

Structure of sumoylated PCNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-147504 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen; Ubiquitin-like protein SMT3 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.63 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12306 (Residues: 1-98; Coverage: 97%)
  • Canonical: P15873 (Residues: 165-258; Coverage: 36%)
Gene names: D9719.15, POL30, SMT3, YBR0811, YBR088C, YDR510W
Sequence domains:
Structure domains:
Proliferating cell nuclear antigen Chain: B
Molecule details ›
Chain: B
Length: 171 amino acids
Theoretical weight: 19.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 1-163; Coverage: 63%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: F432
Unit cell:
a: 268.816Å b: 268.816Å c: 268.816Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.253
Expression system: Escherichia coli