› 3pha

X-ray diffraction
2.17Å resolution

The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose

Released:
DOI: 10.2210/pdb3pha/pdb
PDB entry 3pha coloured by chain, front view.
PDB entry 3pha coloured by chain, side view.
PDB entry 3pha coloured by chain, top view.
Source organism: Blautia obeum ATCC 29174
Entry authors: Tan K, Tesar C, Keigher L, Babnigg G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycosyl hydrolase, family 31 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 666 amino acids
Theoretical weight: 77.43 KDa
Source organism: Blautia obeum ATCC 29174
Expression system: Escherichia coli
UniProt:
  • Canonical: A5ZY13 (Residues: 1-663; Coverage: 100%)
Gene name: RUMOBE_03919
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, AC1
Ligand GLC 8 x GLC
Ligand AC1 4 x AC1
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 88.032Å b: 125.955Å c: 133.58Å
α: 90° β: 107.72° γ: 90°
R-values:
R R work R free
0.203 0.2 0.265
Expression system: Escherichia coli

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