› 3pju

X-ray diffraction
2.5Å resolution

Structure of Pseudomonas fluorescence LapD EAL domain complexed with c-di-GMP, P6522

Released:
DOI: 10.2210/pdb3pju/pdb
PDB entry 3pju coloured by chain, front view.
PDB entry 3pju coloured by chain, side view.
PDB entry 3pju coloured by chain, top view.
Source organism: Pseudomonas fluorescens Pf0-1
Primary publication:
Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis.
Navarro MV, Newell PD, Krasteva PV, Chatterjee D, Madden DR, O'Toole GA, Sondermann H
OpenAccess logo PLoS Biol 9 e1000588 (2011)
PMID: 21304926

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diguanylate cyclase/phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.29 KDa
Source organism: Pseudomonas fluorescens Pf0-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3KK31 (Residues: 400-648; Coverage: 38%)
Gene names: Pfl01_0131, lapD
Sequence domains: EAL domain
Structure domains: EAL domain

Ligands and Environments

1 bound ligand:
Ligand C2E 1 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6522
Unit cell:
a: 141.486Å b: 141.486Å c: 111.254Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.225
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
25 more

6 mentions without citation

Sensing the messenger: the diverse ways that bacteria signal through c-di-GMP.
Krasteva et al. (2012)
5 more